Three-Dimensional Imaging by Confocal Laser Scanning Microscopy(CLSM).
نویسندگان
چکیده
منابع مشابه
Three-dimensional imaging of the intact mouse cochlea by fluorescent laser scanning confocal microscopy.
The complex anatomy of the mammalian cochlea is most readily understood by representation in three-dimensions. However, the cochlea is often sectioned to minimize the effects of its anatomic complexity and optical properties on image acquisition by light microscopy. We have found that optical aberrations present in the decalcified cochlea can be greatly reduced by dehydration through graded eth...
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Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photos...
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The objective of this study was to locate the position of attached or entrapped Salmonella cells in poultry skin. Confocal scanning laser microscopy (CSLM) was used to obtain optical sections of intact poultry skin without artefacts associated with dehydration and other sample preparation techniques. A technique was developed to prevent compression of the poultry skin during CSLM operation. Ima...
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ژورنال
عنوان ژورنال: Journal of Advanced Science
سال: 2001
ISSN: 1881-3917,0915-5651
DOI: 10.2978/jsas.13.7